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Bacteroides, the prominent bacteria in the human gut, play a crucial role in degrading complex polysaccharides. Their abundance is influenced by phages belonging to theCrassviralesorder. Despite identifying over 600Crassviralesgenomes computationally, only few have been successfully isolated. Continued efforts in isolation of moreCrassviralesgenomes can provide insights into phage-host-evolution and infection mechanisms. We focused on wastewater samples, as potential sources of phages infecting variousBacteroideshosts. Sequencing, assembly, and characterization of isolated phages revealed 14 complete genomes belonging to three novelCrassviralesspecies infectingBacteroides cellulosilyticusWH2. These species,Kehishuvirussp. ‘tikkala’ strain Bc01,Kolpuevirussp. ‘frurule’ strain Bc03, and ‘Rudgehvirus jaberico’ strain Bc11, spanned two families, and three genera, displaying a broad range of virion productions. Upon testing all successfully culturedCrassviralesspecies and their respective bacterial hosts, we discovered that they do not exhibit co-evolutionary patterns with their bacterial hosts. Furthermore, we observed variations in gene similarity, with greater shared similarity observed within genera. However, despite belonging to different genera, the three novel species shared a unique structural gene that encodes the tail spike protein. When investigating the relationship between this gene and host interaction, we discovered evidence of purifying selection, indicating its functional importance. Moreover, our analysis demonstrated that this tail spike protein binds to the TonB-dependent receptors present on the bacterial host surface. Combining these observations, our findings provide insights into phage-host interactions and present threeCrassviralesspecies as an ideal system for controlled infectivity experiments on one of the most dominant members of the human enteric virome.

 

As additive manufacturing becomes an increasingly popular method for advanced manufacturing of components, there are many questions that need to be answered before these parts can be implemented for structural purposes. One of the most common concerns with additively manufactured parts is the reliability when subjected to cyclic loadings which has been shown to be highly sensitive to defects such as pores and lack of fusion between layers. It stands to reason that larger parts will inherently have more defects than smaller parts which may result in some sensitivity to surface area differences between these parts. In this research, Ti-6Al-4V specimens with various sizes were produced via a laser-based powder bed fusion method. Uniaxial fatigue tests based on ASTM standards were conducted to generate fatigue-life curves for comparison. Fractography on the fractured specimens was performed to distinguish failure mechanisms between specimen sets with different sizes.